Malic enzyme : Its purification and characterization from Mucor circinelloides and occurrence in other oleaginous fungi
作者： J. Savitha , J. P. Wynn , C. Ratledge
DOI: 10.1007/BF02770799
关键词:
摘要: Malic enzyme was purified 43-fold from Mucor circinelloides. The dependent on Mg2+ or Mn2+ for activity, not active with Dmalate and had a pH optimum at 7.8. apparent Km values malate NADP+ were 488 ΜM 41 Μm respectively. Mr of the native 160 kDa. Five metabolic analogues malate: oxaloacetate, tartronic acid, 1-methylenecyclopropane trans-2,3-dicarboxyIic malonic acid glutaric found to inhibit malic activity 10 mM. Four oleaginous fungi, circinelloides, Mortierella alpina, elongata Pythium ultimum, also examined, all possessed soluble enzyme, two microsomal enzyme.
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