Selecting inocula for the biodegradation of organic compounds at low concentrations.

摘要: The inability of many organisms to degrade pollutants at low concentrations is a problem when selecting inocula for bioremediation sites with these concentrations. Thus, study was conducted determine the effect p-nitrophenol (PNP) on growth four PNP-degrading bacteria and their abilities metabolize compound in culture samples from an oligotrophic lake. PNP did not increase rates Flavobacterium sp. M4, Pseudomonas K, M1, SP3 less than 2, 4, 10, 100 ng/ml, respectively, it sole added carbon source culture, but stimulated multiplication higher In liquid nitro as source, extensively mineralized 50 three degraded much substrate 25 ng/ml. more 20% two strains converted 10% C02 10 none 5% 1 5 ng PNP/ml. Under conditions where 99% radioactivity 14C-PNP ng/ml remained solution, isolates formed organic products. K had no activity 1, 5, contrast, each separately inoculated into water lake well which biodegraded, were able mineralize little addition salts solution glucose per ml resulted mineralization too be carbon. Bacteria may thus substrates natural waters below those suggested by tests media, possibly because availability other sources bacteria.