Gene expression of lactobacilli in murine forestomach biofilms

摘要: Lactobacilli populate the gastro-intestinal tract of vertebrates, and are used in food fermentations as probiotics. also major constituents stable biofilms forestomach rodents. In order to investigate lifestyle these biofilm lactobacilli C57BL/6 mice, we applied metatranscriptomics analyse gene expression (assessed by mRNA) community composition rRNA). Lactobacillales were inhabitants (62–82% rRNA reads), followed Clostridiales (8–31% reads). To identify mRNA transcripts specific for forestomach, compared hindgut metatranscriptomes. Gene microbiota was characterized high abundance related glucose maltose utilization, peptide degradation, amino acid transport, indicating their catabolic anabolic pathways. The transcribed genes encoding pathways enhancing oxidative stress (glutathione synthesis) tolerance. Various pathways, including metabolite formation (urea arginine pathway, γ-aminobutyrate) cell wall modification (DltA, cyclopropane-fatty-acyl-phospholipid synthase), contributed tolerance, judged from transcript profile. addition, expressed numerous extracellular proteins involved adhesion and/or (e.g. MucBP, glycosyl hydrolase families 68 70). This study shed light on adaptations murine that might be relevant other humans.