Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.

摘要: Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% value Brazilian agricultural production, generating a revenue US$6 billion. Despite importance production in Brazil, it supported by narrow genetic base, with few accessions. Molecular differentiation and diversity breeding program were assessed gSSR EST-SSR markers. The study comprised 24 accessions according to their origin: arabica (six traditional genotypes C. arabica), resistant leaf rust-resistant introgression Hibrido de Timor), robusta (five canephora genotypes), Timor (three x canephora), triploids racemosa), racemosa (one racemosa). Allele polymorphism analysis, AMOVA, Student t-test, Jaccard's dissimilarity coefficient, cluster correlation distances, discriminant performed. markers gave 25 exclusive alleles per group, while showed 47, which will be useful differentiating fingerprinting varieties. detected higher percentage among (35% on average) within (42.9% groups, compared highest groups was found (89.2%) (39.5%). It possible differentiate all including arabica-related Nevertheless, combined use recommended molecular characterization, because EST-SSRs can provide complementary information.