The Nairovirus Nairobi Sheep Disease Virus/Ganjam Virus Induces the Translocation of Protein Disulphide Isomerase-Like Oxidoreductases from the Endoplasmic Reticulum to the Cell Surface and the Extracellular Space

摘要: Nairobi sheep disease virus (NSDV) of the genus Nairovirus causes a haemorrhagic gastroenteritis in and goats with mortality up to 90%; is found East Central Africa, India, where called Ganjam virus. NSDV closely related human pathogen Crimean-Congo fever virus, which also disease. As other nairoviruses, replication takes place cytoplasm new particles bud into Golgi apparatus; however, effect viral on cellular compartments has not been studied extensively. We have that overall structure endoplasmic reticulum (ER), ER-Golgi intermediate compartment were unaffected by infection NSDV. However, we observed led loss protein disulphide isomerase (PDI), an oxidoreductase present lumen (ER) assists during folding, from ER. Further investigation showed NSDV-infected cells high levels PDI at their surface, secreted culture medium infected cells. Another chaperone family, ERp57, was be similarly affected. Analysis expression individual glycoproteins indicated PreGn glycoprotein involved redistribution these soluble ER oxidoreductases. It suggested extracellular can activate integrins tissue factor, are respectively pro-inflammatory responses disseminated intravascular coagulation, both manifest many fevers. The discovery enhanced secretion may important finding for understanding mechanisms underlying pathogenicity nairoviruses.