摘要: Microcrystal electron diffraction, or MicroED, is a method that capable of determining structure from very small and thin 3D crystals using transmission microscope. MicroED has been successfully used on microcrystalline samples, including proteins, peptides, organic molecules, in many cases to high resolutions. In this work, the workflow will be briefly described areas future development highlighted. These include improvements sample preparation, data collection, determination.