MicroED methodology and development.

作者: Brent L. Nannenga

DOI: 10.1063/1.5128226

关键词:

摘要: Microcrystal electron diffraction, or MicroED, is a method that capable of determining structure from very small and thin 3D crystals using transmission microscope. MicroED has been successfully used on microcrystalline samples, including proteins, peptides, organic molecules, in many cases to high resolutions. In this work, the workflow will be briefly described areas future development highlighted. These include improvements sample preparation, data collection, determination.

参考文章(106)
Tamir Gonen, The collection of high-resolution electron diffraction data. Methods of Molecular Biology. ,vol. 955, pp. 153- 169 ,(2013) , 10.1007/978-1-62703-176-9_9
J. M. Zuo, M. Kim, M. O'Keeffe, J. C. H. Spence, Direct observation of d-orbital holes and Cu-Cu bonding in Cu2O Nature. ,vol. 401, pp. 49- 52 ,(1999) , 10.1038/43403
Johan Hattne, Francis E. Reyes, Brent L. Nannenga, Dan Shi, M. Jason de la Cruz, Andrew G. W. Leslie, Tamir Gonen, MicroED data collection and processing Acta Crystallographica Section A. ,vol. 71, pp. 353- 360 ,(2015) , 10.1107/S2053273315010669
George M. Sheldrick, Crystal structure refinement with SHELXL Acta Crystallographica Section C-crystal Structure Communications. ,vol. 71, pp. 3- 8 ,(2015) , 10.1107/S2053229614024218
U. Kolb, T. Gorelik, C. Kübel, M.T. Otten, D. Hubert, Towards automated diffraction tomography: part I--data acquisition. Ultramicroscopy. ,vol. 107, pp. 507- 513 ,(2007) , 10.1016/J.ULTRAMIC.2006.10.007
Werner Kühlbrandt, Da Neng Wang, Yoshinori Fujiyoshi, Atomic model of plant light-harvesting complex by electron crystallography Nature. ,vol. 367, pp. 614- 621 ,(1994) , 10.1038/367614A0
N. Grigorieff, T.A. Ceska, K.H. Downing, J.M. Baldwin, R. Henderson, Electron-crystallographic refinement of the structure of bacteriorhodopsin. Journal of Molecular Biology. ,vol. 259, pp. 393- 421 ,(1996) , 10.1006/JMBI.1996.0328
Enrico Mugnaioli, Iryna Andrusenko, Timo Schüler, Niklas Loges, Robert E. Dinnebier, Martin Panthöfer, Wolfgang Tremel, Ute Kolb, Ab Initio Structure Determination of Vaterite by Automated Electron Diffraction Angewandte Chemie. ,vol. 51, pp. 7041- 7045 ,(2012) , 10.1002/ANIE.201200845
Shijun Zhong, Voichita M. Dadarlat, Robert M. Glaeser, Teresa Head-Gordon, Kenneth H. Downing, Modeling chemical bonding effects for protein electron crystallography: the transferable fragmental electrostatic potential (TFESP) method Acta Crystallographica Section A. ,vol. 58, pp. 162- 170 ,(2002) , 10.1107/S0108767301020256