Are genuine changes in protein expression being overlooked? Reassessing Western blotting

摘要: This study used purified calsequestrin 1 and AMP kinase (AMPK) proteins to demonstrate how Western blotting outcomes can be influenced when either the density of detected lie within a nonproportional region standard curve or is not taken into account for data analyses. It outlines likelihood true changes being overlooked through simple mistake using band alone and/or analyzing too much sample. To this, extrapolation typical linear, although nonproportional, resulted in approximately fourfold error. The method was estimate concentration AMPK β1 rat extensor digitorum longus muscle as order 60 μM. article suggests that adopting more sensitive protocol will improve reliability quantitative outcomes.