Interleukin-8 in Hodgkin's disease. Preferential expression by reactive cells and association with neutrophil density.

摘要: Hodgkin's disease (HD) shows rare neoplastic Hodgkin and Reed-Sternberg cells embedded in an abundant reactive infiltrate containing, among other cell types, neutrophilic granulocytes. Interleukin (IL)-8 is chemotactic for neutrophils. The expression of IL-8 was tested by situ hybridization with 35S-labeled IL-8-specific RNA probes on 38 cases HD. Reactive lesions, non-Hodgkin's lymphomas B T phenotype, Langerhans histiocytosis served as controls. observed 3 33 classical HD 20 evidenced combined isotopic immunohistology the demonstration cell-type-characteristic antigens or enzyme histochemistry chloroacetate esterase. IL-8-positive were more numerous nodular sclerosing compared mixed cellularity histotype (P = 0.01). number density neutrophils positively correlated < 0. 01). In 5 lymphocyte-predominant HD, not displayed. Non-Hodgkin's lymphoma contained transcripts only 1 23 sparse cells. 4 7 histiocytosis, signals displayed S100-negative conclusion, largely confined to associated infiltration Elaboration cytokines may explain frequent this cytokine particularly type.