Crystallization and preliminary X-ray diffraction analysis of the two distinct types of zebrafish β2-microglobulin.

摘要: β(2)-Microglobulin (β(2)m) noncovalently associates with the heavy chain of major histocompatibility complex class I (MHC I) molecules, which bind foreign antigen peptides to control cytotoxic T lymphocyte (CTL) immune response. In contrast mammals, there are distinct types β(2)ms derived from two loci in a number teleost species. order clarify structures β(2)ms, zebrafish (Danio rerio) Dare-β(2)m-I and Dare-β(2)m-II were expressed Escherichia coli, purified crystallized, diffraction data collected 1.6 1.9 A resolution, respectively. Both crystals belonged space group P2(1)2(1)2(1). The unit-cell parameters determined be = 38.2, b 50.4, c 50.9 for 38.9, 52.7, 65.8 Dare-β(2)m-II. Each asymmetric unit was constituted one molecule, Matthews coefficients 2.22 3.01 A(3) Da(-1) solvent contents 45 59% Dare-β(2)m-II, These β(2)m will provide relevant information further studies MHC complex.