Theranostic verteporfin- loaded lipid-polymer liposome for photodynamic applications.
作者： Daphne Christine Salles de Oliveira , Camila Fabiano de Freitas , Italo Rodrigo Calori , Renato Sonchini Goncalves , Camila Aparecida Errerias Fernandes Cardinali
DOI: 10.1016/J.JPHOTOBIOL.2020.112039
关键词:
摘要: Abstract In this study we report a novel theranostic lipid-polymer liposome, obtained from DPPC and the triblock copolymer F127 covalently modified with 5(6)-carboxyfluorescein (CF) for photodynamic applications. Due to presence of F127, small unilamellar vesicle (SUV) liposomes were synthesized by simple fast thin-film hydration method without need an extrusion process. The vesicles have around 100 nm, low polydispersity superb solution stability. clinically used photosensitizer verteporfin (VP) was entrapped into vesicles, mostly in monomeric form, 90% loading efficiency. Stern-Volmer fluorescence lifetime assays showed heterogeneous distribution VP CF ensuring integrity their individual photophysical properties. properties entirely photoactivatable can be trigged unique wavelength (470 nm). feasibility system tested against Glioblastoma multiforme cell line T98G. Cellular uptake time-resolved microscopy monomerized (monoexponential decay, 6.0 ns) at nucleus level, while detected membrane microscopy. strategy's success supported reduction 98% viability T98G cells photoactivated liposome [VP] = 1.0 μmol L−1. Therefore, is potential use cancer ocular disease therapies.
sci-hub.st 参考文章(66)
Dean W. Felsher, Cancer revoked: oncogenes as therapeutic targets Nature Reviews Cancer. ,vol. 3, pp. 375- 379 ,(2003) , 10.1038/NRC1070
Raymond F. Chen, Jay R. Knutson, Mechanism of fluorescence concentration quenching of carboxyfluorescein in liposomes: energy transfer to nonfluorescent dimers. Analytical Biochemistry. ,vol. 172, pp. 61- 77 ,(1988) , 10.1016/0003-2697(88)90412-5
Nitin A. Bhimte, Pralhad T. Tayade, Evaluation of microcrystalline cellulose prepared from sisal fibers as a tablet excipient: a technical note. Aaps Pharmscitech. ,vol. 8, pp. 8- ,(2007) , 10.1208/PT0801008
Yogeshkumar Malam, Marilena Loizidou, Alexander M. Seifalian, Liposomes and nanoparticles: nanosized vehicles for drug delivery in cancer. Trends in Pharmacological Sciences. ,vol. 30, pp. 592- 599 ,(2009) , 10.1016/J.TIPS.2009.08.004
T.M. Allen, A. Chonn, Large unilamellar liposomes with low uptake into the reticuloendothelial system FEBS Letters. ,vol. 223, pp. 42- 46 ,(1987) , 10.1016/0014-5793(87)80506-9
Noboru Hioka, Fernanda Ibanez Simplicio, Florângela Maionchi, TERAPIA FOTODINÂMICA: ASPECTOS FARMACOLÓGICOS, APLICAÇÕES E AVANÇOS RECENTES NO DESENVOLVIMENTO DE MEDICAMENTOS Química Nova. ,vol. 25, pp. 801- 807 ,(2002) , 10.1590/S0100-40422002000500016
Jun Fang, Hideaki Nakamura, Hiroshi Maeda, The EPR effect: Unique features of tumor blood vessels for drug delivery, factors involved, and limitations and augmentation of the effect Advanced Drug Delivery Reviews. ,vol. 63, pp. 136- 151 ,(2011) , 10.1016/J.ADDR.2010.04.009
Gregor Cevc, Rational design of new product candidates: the next generation of highly deformable bilayer vesicles for noninvasive, targeted therapy. Journal of Controlled Release. ,vol. 160, pp. 135- 146 ,(2012) , 10.1016/J.JCONREL.2012.01.005
Minakshi Garg, Tathagata Dutta, Narendra K Jain, None, Stability study of stavudine-loaded O-palmitoyl-anchored carbohydrate-coated liposomes Aaps Pharmscitech. ,vol. 8, ,(2007) , 10.1208/PT0802038
Kaavya A. Mohanasundaram, Naomi L. Haworth, Mani P. Grover, Tamsyn M. Crowley, Andrzej Goscinski, Merridee A. Wouters, Potential role of glutathione in evolution of thiol-based redox signaling sites in proteins. Frontiers in Pharmacology. ,vol. 6, pp. 1- 15 ,(2015) , 10.3389/FPHAR.2015.00001