Steady-state and dynamic gene expression programs in Saccharomyces cerevisiae in response to variation in environmental nitrogen

摘要: Cell growth rate is regulated in response to the abundance and molecular form of essential nutrients. InSaccharomyces cerevisiae(budding yeast), environmental nitrogen a major determinant cell rate, supporting rates that vary at least threefold. Transcriptional control use mediated large part by catabolite repression (NCR), which results specific transcripts presence preferred source supports fast such as glutamine, are otherwise expressed nonpreferred source, proline, slower rate. Differential expression NCR regulon additional nitrogen-responsive genes >500 differentially cells growing different sources batch cultures. Here we find rate-controlled cultures using nitrogen-limited chemostats, gene programs strikingly similar regardless source. derepressed all nitrogen-limiting chemostat conditions these conditions, only 34 exhibit source-specific differential expression. Addition either or chemostats rapid, dose-dependent regulon. Using novel means computational normalization compare global steady-state dynamic evidence addition transient overproduction required for protein translation. Simultaneously, accelerated mRNA degradation underlies rapid clearing subset transcripts, most pronounced highly NCR-regulated permease genesGAP1,MEP2,DAL5,PUT4, andDIP5 Our reveal aspects nitrogen-regulated highlight need quantitative approach study how coordinates translation assimilation optimize environments.