A primer for morpholino use in zebrafish.
作者： Brent R. Bill , Andrew M. Petzold , Karl J. Clark , Lisa A. Schimmenti , Stephen C. Ekker
关键词:
摘要: Abstract Morpholino oligonucleotides are the most common anti-sense “knockdown” technique used in zebrafish (Danio rerio). This review discusses practices for design, preparation, and deployment of morpholinos this vertebrate model system. Off-targeting effects discussed as well method to minimize potentially confounding variable via co-injection a tP53-targeting morpholino. Finally, new uses summarized contextualized with respect complementary, DNA-based knockout technologies recently developed zebrafish.
elsevier.com
liebertonline.com参考文章(49)
Stephen C. Ekker, Nonconventional Antisense in Zebrafish for Functional Genomics Applications Methods in Cell Biology. ,vol. 77, pp. 121- 136 ,(2004) , 10.1016/S0091-679X(04)77007-7
Cathleen Teh, Shang Wei Chong, Vladimir Korzh, DNA delivery into anterior neural tube of zebrafish embryos by electroporation. BioTechniques. ,vol. 35, pp. 950- 954 ,(2003) , 10.2144/03355ST02
Tana M. Hyatt, Stephen C. Ekker, Vectors and techniques for ectopic gene expression in zebrafish. Methods in Cell Biology. ,vol. 59, pp. 117- 126 ,(1998) , 10.1016/S0091-679X(08)61823-3
Monte Westerfield, The zebrafish book : a guide for the laboratory use of zebrafish (Danio rerio) M. Westerfield. ,(1995)
Eric Wickstrom, Karen A. Urtishak, Michael Choob, Xiaobing Tian, Nitzan Sternheim, Laura M. Cross, Amy Rubinstein, Steven A. Farber, Downregulation of gene expression with negatively charged peptide nucleic acids (PNAs) in zebrafish embryos. Methods in Cell Biology. ,vol. 77, pp. 137- 158 ,(2004) , 10.1016/S0091-679X(04)77008-9
Monte Westerfield, Eckehard Doerry, Arthur E. Kirkpatrick, Sarah A. Douglas, Zebrafish informatics and the ZFIN database. Methods in Cell Biology. ,vol. 60, pp. 339- 355 ,(1998) , 10.1016/S0091-679X(08)61909-3
M. Furutani-Seiki, C. Nusslein-Volhard, R. N. Kelsh, C.-P. Heisenberg, F. Pelegri, J. Odenthal, M. Granato, D. A. Kane, M. C. Mullins, F. J. M. Van Eeden, R. M. Warga, M. Brand, Y.-J. Jiang, P. Haffter, M. Hammerschmidt, dino and mercedes, two genes regulating dorsal development in the zebrafish embryo Development. ,vol. 123, pp. 95- 102 ,(1996) , 10.1242/DEV.123.1.95
Jeffrey D Amack, H.Joseph Yost, The T Box Transcription Factor No Tail in Ciliated Cells Controls Zebrafish Left-Right Asymmetry Current Biology. ,vol. 14, pp. 685- 690 ,(2004) , 10.1016/J.CUB.2004.04.002
Michael A. Pickart, Eric W. Klee, Aubrey L. Nielsen, Sridhar Sivasubbu, Eric M. Mendenhall, Brent R. Bill, Eleanor Chen, Craig E. Eckfeldt, Michelle Knowlton, Mara E. Robu, Jon D. Larson, Yun Deng, Lisa A. Schimmenti, Lynda B.M. Ellis, Catherine M. Verfaillie, Matthias Hammerschmidt, Steven A. Farber, Stephen C. Ekker, Genome-Wide Reverse Genetics Framework to Identify Novel Functions of the Vertebrate Secretome PLOS ONE. ,vol. 1, ,(2006) , 10.1371/JOURNAL.PONE.0000104
Brent R. Bill, Darius Balciunas, Joshua A. McCarra, Eric D. Young, Toua Xiong, Ashley M. Spahn, Marta Garcia-Lecea, Vladimir Korzh, Stephen C. Ekker, Lisa A. Schimmenti, Development and Notch signaling requirements of the zebrafish choroid plexus. PLOS ONE. ,vol. 3, ,(2008) , 10.1371/JOURNAL.PONE.0003114