Metabolic changes associated to the unblocking of adventitious root formation in aged, rooting-recalcitrant cuttings of Eucalyptus gunnii Hook. f. (Myrtaceae)

摘要: In many tree species, the ability to produce adventitious roots is limited by specific changes related physiology of reproduction and ageing. spite its ecological significance, little known about physiological mechanisms determining irreversibility these non-rooting states in some species. this study, we performed natural hormone-induced rooting stem cuttings from mature individuals rooting-recalcitrant Eucalyptus gunnii order characterize poorly metabolic associated formation a Stem cuttings, either with or without an apical meristem, were put greenhouse after having been treated two different synthetic auxins, indole-3-butyric acid 1-naphthaleneacetic acid. During processes secondary metabolism cutting tissue observed analyzed through chromatographic techniques. Rooting E. proved be hastened accumulation tissues methyl gallate. Its metabolization disappearance during long period stay benches restored adventive ability. same period, concomitant quercetin-like flavonoids was observed. enhanced addition acid, although meristem not influenced hormonal treatments. Seasonal conditions had no effect on percentage rooted took 1 month longer autumn/winter trial. general, auxinic treatments did affect production new leaves but favor initial differentiation root tissue. blocked presence inhibitor gallate, degradation which allowed take place. Metabolization further glycosylation flavonols. Our results suggest that switch between pathways gallic shikimic other flavonoid precursors crucial step process leading aged cuttings.