Zeta method: A noninvasive method based on membrane charge for selecting spermatozoa expressing high level of phospholipaseCζ.

摘要: Intracytoplasmic sperm injection (ICSI) is a technique developed to help attain successful fertilisation for couples with severe male factor. However, small percentage of confront low or failed fertilisation, mainly due oocyte activation. Several studies have introduced phospholipase Cζ (PLCζ) as the main factor inducing activation and thereby has potential act biomarker prediction ICSI outcome. On other hand, researchers focused on novel selection procedures based cellular characteristics spermatozoa such surface electrical charge (Zeta potential) isolate normal subpopulation intact chromatin. Therefore, we aimed compare PLCζ between Zeta method routine preparation procedure: density gradient centrifugation (DGC). Our results showed that number PLCζ-positive was significantly in method, but intensity protein higher than DGC procedure. combination procedure may allow selecting population high which also contain amount This can open new approach infertile men previously fertilisation.