Molecular sexing of African rhinoceros.
作者： Lindsay Peppin , Ross McEwing , Rob Ogden , Robert Hermes , Cindy Harper
DOI: 10.1007/S10592-009-9912-2
关键词:
摘要: We report the development of a fast and reliable PCR-based method for sex identification African Rhinoceros (Ceratotherium simum Diceros bicornis) that could easily be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting fluorescently labelled forward an unlabelled reverse primer, is used to co-amplify homologous fragments zinc finger (ZF) protein intron which exhibits size polymorphism between X Y chromosomes. In both species, amplified ZFX ZFY amplicons differ in by 7 bp can thus differentiated capillary electrophoresis. Blood, tissue, horn, faecal samples were correctly sexed using this method. Cross species testing also demonstrated Indian rhinoceros (Rhinoceros unicornis) samples.
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sci-hub.se 参考文章(10)
John F Dallas, David N Carss, Freda Marshall, Klaus-Peter Koepfli, Hans Kruuk, Philip J Bacon, Stuart B Piertney, Sex identification of the Eurasian otter Lutra lutra by PCR typing of spraints Conservation Genetics. ,vol. 1, pp. 181- 183 ,(2000) , 10.1023/A:1026551510861
David Arnold Hill, None, Handbook of Biodiversity Methods: Survey, Evaluation and Monitoring ,(2005)
Palle Villesen, Tina Fredsted, A new sex identification tool: One primer pair can reliably sex ape and monkey DNA samples Conservation Genetics. ,vol. 7, pp. 455- 459 ,(2006) , 10.1007/S10592-005-9044-2
Joel Berger, Predation, sensitivity, and sex: why female black rhinoceroses outlive males Behavioral Ecology. ,vol. 6, pp. 57- 64 ,(1995) , 10.1093/BEHECO/6.1.57
P. TABERLET, H. MATTOCK, C. DUBOIS-PAGANON, J. BOUVET, Sexing free-ranging brown bears Ursus arctos using hairs found in the field. Molecular Ecology. ,vol. 2, pp. 399- 403 ,(1993) , 10.1111/J.1365-294X.1993.TB00033.X
R. AMIN, K. THOMAS, R. H. EMSLIE, T. J. FOOSE, N. VAN STRIEN, An overview of the conservation status of and threats to rhinoceros species in the wild International Zoo Yearbook. ,vol. 40, pp. 96- 117 ,(2006) , 10.1111/J.1748-1090.2006.00096.X
Mark J Statham, Peter D Turner, Catherine O'Reilly, Molecular Sex Identification of Five Mustelid Species Zoological Studies. ,vol. 46, pp. 600- 608 ,(2007)
Matthew E. Durnin, Per J. Palsbøll, Oliver A. Ryder, Dale R. McCullough, A reliable genetic technique for sex determination of giant panda (Ailuropoda melanoleuca) from non-invasively collected hair samples Conservation Genetics. ,vol. 8, pp. 715- 720 ,(2007) , 10.1007/S10592-006-9196-8
Steinar Engen, Russell Lande, Bernt-Erik SÆther, DEMOGRAPHIC STOCHASTICITY AND ALLEE EFFECTS IN POPULATIONS WITH TWO SEXES Ecology. ,vol. 84, pp. 2378- 2386 ,(2003) , 10.1890/02-0123
Xiao Xu, Ling Lin, Zhihe Zhang, Fujun Shen, Liang Zhang, Bisong Yue, A reliable, non-invasive PCR method for giant panda (Ailuropoda melanoleuca) sex identification Conservation Genetics. ,vol. 9, pp. 739- 741 ,(2008) , 10.1007/S10592-007-9376-1