Partial Characterization of a cDNA for human stearoyl-CoA desaturase and changes in its mRNA expression in some normal and malignant tissues.
作者： Juan Li , Shi-Fa Ding , Nagy A. Habib , Beverley F. Fermor , Chris B. Wood
关键词:
摘要: A segment of 712 bases coding for part the human stearoyl-CoA desaturase gene was made by polymerase chain reaction (PCR) using primers based on published rat cDNA sequences. The PCR product confirmed DNA sequencing. It next cloned into a vector from which anti-sense, highly radioactive RNA transcripts were in vitro T7 polymerase. used to probe mRNA number tumour and control tissues, very sensitive solution hybridization/RNase protection assay. Increased levels found colonic oesophageal carcinomas hepatocellular adenoma; however, no consistent trend seen carcinoma. is suggested that certain classes may exhibit increased mRNA. © 1994 Wiley-Liss, Inc.
sci-hub.se 参考文章(14)
J M Ntambi, S A Buhrow, K H Kaestner, R J Christy, E Sibley, T J Kelly, M D Lane, Differentiation-induced gene expression in 3T3-L1 preadipocytes. Characterization of a differentially expressed gene encoding stearoyl-CoA desaturase. Journal of Biological Chemistry. ,vol. 263, pp. 17291- 17300 ,(1988) , 10.1016/S0021-9258(19)77834-X
M A Thiede, J Ozols, P Strittmatter, Construction and sequence of cDNA for rat liver stearyl coenzyme A desaturase. Journal of Biological Chemistry. ,vol. 261, pp. 13230- 13235 ,(1986) , 10.1016/S0021-9258(18)69295-6
K H Kaestner, J M Ntambi, T J Kelly, M D Lane, Differentiation-induced gene expression in 3T3-L1 preadipocytes: a second differentially expressed gene encoding stearoyl-CoA desaturase Journal of Biological Chemistry. ,vol. 264, pp. 14755- 14761 ,(1989) , 10.1016/S0021-9258(18)63763-9
Per Isaksson, Harold Newmark, Brigitte Marian, Sidney Winawer, Eileen Friedman, Joseph Rafter, Fecal diglycerides as selective endogenous mitogens for premalignant and malignant human colonic epithelial cells Cancer Research. ,vol. 49, pp. 544- 548 ,(1989)
DE Khoo, B Fermor, J Miller, CB Wood, K Apostolov, W Barker, RCN Williamson, NA Habib, Manipulation of body fat composition with sterculic acid can inhibit mammary carcinomas in vivo. British Journal of Cancer. ,vol. 63, pp. 97- 101 ,(1991) , 10.1038/BJC.1991.20
Osamu Doi, Fumiko Doi, Friedhelm Schroeder, Alfred W. Alberts, P.Roy Vagelos, Manipulation of fatty acid composition of membrane phospholipid and its effects on cell growth in mouse LM cells Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 509, pp. 239- 250 ,(1978) , 10.1016/0005-2736(78)90044-5
David E. Wennerstrom, Howard M. Jenkin, The effect of two isomeric octadecenoic acids on the lipid metabolism and growth of Novikoff hepatoma cells. Biochimica et Biophysica Acta. ,vol. 431, pp. 469- 480 ,(1976) , 10.1016/0005-2760(76)90213-7
Shigenobu Umeki, Hirofumi Fukushima, Yoshinori Nozawa, Variations in the activity of fatty ACYL-CoA desaturases and electron-transport components in Tetrahymena microsomes with temperature and age of culture Journal of Thermal Biology. ,vol. 8, pp. 353- 360 ,(1983) , 10.1016/0306-4565(83)90022-0
Beverley F Fermor, John R.W Masters, Christopher B Wood, Jayne Miller, Kosta Apostolov, Nagy A Habib, Fatty acid composition of normal and malignant cells and cytotoxicity of stearic, oleic and sterculic acids in vitro. European Journal of Cancer. ,vol. 28, pp. 1143- 1147 ,(1992) , 10.1016/0959-8049(92)90475-H
RP Gleeson, M Ayub, JT Wright, CB Wood, NA Habib, WP Soutter, MHF Sullivan, JO White, Fatty acid control of growth of human cervical and endometrial cancer cells. British Journal of Cancer. ,vol. 61, pp. 500- 503 ,(1990) , 10.1038/BJC.1990.113