p47phox Deficiency Impairs NF-κB Activation and Host Defense in Pseudomonas Pneumonia
作者： Ruxana T Sadikot , Heng Zeng , Fiona E Yull , Bo Li , Dong-sheng Cheng
DOI: 10.4049/JIMMUNOL.172.3.1801
关键词:
摘要: We examined the role of redox signaling generated by NADPH oxidase in activation NF-kappaB and host defense against Pseudomonas aeruginosa pneumonia. Using mice with an NF-kappaB-driven luciferase reporter construct (HIV-LTR/luciferase (HLL)), we found that intratracheal administration P. resulted a dose-dependent neutrophilic influx NF-kappaB. To determine effects reactive oxygen species system on NF-kappaB, crossbred deficient p47(phox) (p47(phox-/-)HLL). These p47(phox-/-)HLL were unable to activate same degree as HLL intact following infection. In addition, lung TNF-alpha levels significantly lower compared mice. Bacterial clearance was impaired vitro studies using bone marrow-derived macrophages showed Toll-like receptor 4 necessary for treatment aeruginosa. Additional from p47(phox-/-) confirmed maximal 4-dependent this model. data indicate oxidase-dependent respiratory burst stimulated infection contributes modulating redox-dependent through pathway.
jimmunol.org参考文章(34)
F Gannon, F Deryckere, A one-hour minipreparation technique for extraction of DNA-binding proteins from animal tissues. BioTechniques. ,vol. 16, pp. 405- ,(1994)
Ruxana T. Sadikot, Wei Han, M. Brett Everhart, Ornella Zoia, R. Stokes Peebles, E. Duco Jansen, Fiona E. Yull, John W. Christman, Timothy S. Blackwell, Selective IκB Kinase Expression in Airway Epithelium Generates Neutrophilic Lung Inflammation Journal of Immunology. ,vol. 170, pp. 1091- 1098 ,(2003) , 10.4049/JIMMUNOL.170.2.1091
T S Blackwell, B W Christman, J W Christman, E P Holden, T R Blackwell, In vivo antioxidant treatment suppresses nuclear factor-kappa B activation and neutrophilic lung inflammation. Journal of Immunology. ,vol. 157, pp. 1630- 1637 ,(1996)
Bernard M. Babior, NADPH Oxidase: An Update Blood. ,vol. 93, pp. 1464- 1476 ,(1999) , 10.1182/BLOOD.V93.5.1464
Rhodri Ceredig, Matthias Merkenschlager, Vladimir N. Ivanov, Antioxidant treatment of thymic organ cultures decreases NF-kappa B and TCF1(alpha) transcription factor activities and inhibits alpha beta T cell development. Journal of Immunology. ,vol. 151, pp. 4694- 4704 ,(1993) , 10.7916/D85X2873
Gina H. Chen, Raju C. Reddy, Michael W. Newstead, Kazuhiro Tateda, Bhavani L. Kyasapura, Theodore J. Standiford, Intrapulmonary TNF gene therapy reverses sepsis-induced suppression of lung antibacterial host defense. Journal of Immunology. ,vol. 165, pp. 6496- 6503 ,(2000) , 10.4049/JIMMUNOL.165.11.6496
Henry Jay Forman, Martine Torres, Reactive oxygen species and cell signaling: respiratory burst in macrophage signaling. American Journal of Respiratory and Critical Care Medicine. ,vol. 166, ,(2002) , 10.1164/RCCM.2206007
S Frackman, M Anhalt, K H Nealson, Cloning, organization, and expression of the bioluminescence genes of Xenorhabdus luminescens. Journal of Bacteriology. ,vol. 172, pp. 5767- 5773 ,(1990) , 10.1128/JB.172.10.5767-5773.1990
Jie Fan, Randall S. Frey, Arshad Rahman, Asrar B. Malik, Role of Neutrophil NADPH Oxidase in the Mechanism of Tumor Necrosis Factor-α-induced NF-κB Activation and Intercellular Adhesion Molecule-1 Expression in Endothelial Cells Journal of Biological Chemistry. ,vol. 277, pp. 3404- 3411 ,(2002) , 10.1074/JBC.M110054200
Christopher H. Contag, Stanley D. Spilman, Pamela R. Contag, Masafumi Oshiro, Brian Eames, Phyllis Dennery, David K. Stevenson, David A. Benaron, Visualizing gene expression in living mammals using a bioluminescent reporter. Photochemistry and Photobiology. ,vol. 66, pp. 523- 531 ,(1997) , 10.1111/J.1751-1097.1997.TB03184.X