Immunogenetic effects of low dose (CEM43 30) magnetic nanoparticle hyperthermia and radiation in melanoma cells.

摘要: Objective: In this in vitro study we have used an RNA quantification technique, nanoString, and a conventional protein analysis technique (Western Blot) to assess the genetic expression of B16 murine melanoma cells following modest magnetic nanoparticle hyperthermia (mNPH) dose equivalent 30 minutes @ 43°C (CEM43 30) and/or clinically relevant 8 Gy radiation dose.Methods: Melanoma with mNPs(2.5 μg Fe/106 cells) were pelleted exposed alternating field (AMF) generate targeted thermal dose. Thermal was accurately monitored by fiber optic probe automatically maintained at CEM43 30. All harvested 24 hours after treatment.Results: The mNPH demonstrated notable elevations thermotolerance/immunogenic HSP70 gene number chemoattractant toll-like receptor pathways. also upregulated important immune cytotoxic However, mNPH/radiation combination most effective stimulator wide variety genes including HSP70, cancer regulating chemokines CXCL10, CXCL11, T-cell trafficking chemokine CXCR3, innate activators TLR3, TLR4, MDM2 mTOR negative regulator p53, pro-apoptotic PUMA, cell death Fas. Importantly changes validated changes, i.e., p-mTOR, p-MDM2.Conclusion: These results not only show that low independently increase but effect is greatly enhanced when they are combination.