Micropatterning of TCR and LFA-1 ligands reveals complementary effects on cytoskeleton mechanics in T cells.

摘要: The formation of the immunological synapse between a T cell and antigen-presenting (APC) is critically dependent on actin dynamics, downstream receptor (TCR) integrin (LFA-1) signalling. There also accumulating evidence that mechanical forces, generated by polymerization and/or myosin contractility regulate Because both pathways are intertwined, their contributions towards cytoskeletal organization remain elusive. Here, we identify specific roles TCR LFA-1 using combination micropatterning to spatially separate signalling systems nanopillar arrays for high-precision analysis cellular forces. We Arp2/3 acts TCRs nucleate dense foci but propagation network requires formin FHOD1. adhesion enhances actomyosin which in turn modulate assembly TCR. Together our data shows mechanically cooperative system through ligands presented an APC activation.