Analysis of anandamide, an endogenous cannabinoid substance, and of other natural N-acylethanolamines
作者： A. Fontana , V. Di Marzo , H. Cadas , D. Piomelli
DOI: 10.1016/0952-3278(95)90130-2
关键词:
摘要: Recent reports have suggested that N-arachidonoylethanolamine (anandamide) acts as an endogenous ligand for cannabinoid receptors in mammalian brain. Here we describe methods the extraction, purification and analysis of anandamide related N-fatty acyl-ethanolamines (NAEs). Liquid-phase silica gel G column chromatography thin-layer (TLC) were employed sample fractionation. Three analytical high-performance liquid (HPLC) NAEs developed. Finally, analyses by gas chromatography/mass spectrometry (GC/MS) are described. The applications these to identification cell cultures well artifacts biosynthetic studies
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