Duplication and maintenance of the Myb genes of vertebrate animals

摘要: Gene duplication is an important means of generating new genes. The major mechanisms by which duplicated genes are preserved in the face purifying selection thought to be neofunctionalization, subfunctionalization, and increased gene dosage. However, very few families vertebrate species have been analyzed functional tests vivo. We therefore examined three Myb (c-Myb, A-Myb, B-Myb) cytogenetic map analysis, sequence ectopic expression Drosophila. provide evidence that arose two rounds regional genomic duplication. found ubiquitous c-Myb but not B-Myb or Drosophila Myb, was lethal Expression any these during early larval eye development well tolerated. late caused drastic alterations adult morphology. Mosaic analysis implied this phenotype cell-autonomous. Interestingly, some phenotypes retroviral v-Myb oncogene normal proto-oncogene from were quite distinct. Finally, we post-translational modifications GSK-3 protein kinase Ubc9 SUMO-conjugating enzyme normally occur cells can modify These results support a model vertebrates sequential duplications. first followed subfunctionalization expression, then neofunctionalization function yield c/A-Myb progenitor. progenitor give rise tissue-specific A-Myb