Growth-Stimulatory Effect of Quorum Sensing Signal Molecule N-Acyl-Homoserine Lactone-Producing Multi-Trait Aeromonas spp. on Wheat Genotypes Under Salt Stress.

摘要: Salinity is one of the major threats to agricultural productivity worldwide. Soil and plant management practices, along with inoculation plant-beneficial bacteria, play a key role in plant's tolerance toward salinity stress. The present study demonstrates potential acyl homoserine lactone (AHL)-producing growth promoting rhizobacteria (PGPR) strains Aeromonas sp., namely, SAL-17 (accession no. HG763857) SAL-21 HG763858), for promotion two wheat genotypes inherently different salt potential. AHLs are bacterial signal molecules that regulate expression various genes bacteria plants. Both spp., innate plant-growth-promoting (PGP) traits, showed AHL production which was identified on tandem mass spectrometry as C6-HSL, 3-OH-C5-HSL, 3-OH-C6-HSL, 3-oxo-C7-HSL C10-HSL, 3-oxo-C10-HSL, 3-OH-C10-HSL, 3-oxo-C12-HSL 3-oxo-C10-HSL. exogenous application purified (mix) significantly improved root parameters at 200 mM NaCl both salt-sensitive (SSG) salt-tolerant (STG) genotypes, where highest increase (≈80%) observed mixture used. Confocal microscopic observations overlay assay revealed strong colonization under response STG SSG evaluated AHL-producing (SAL-17 SAL-21) compared non-AHL-producing sp. SAL-12 HG763856) saline (EC = 7.63 ms/cm2) non-saline soil. data reveal plants inoculated consortium (SAL-21 + SAL-17) maximum leaf proline content, nitrate reductase activity, chlorophyll a/b, stomatal conductance, transpiration rate, length, shoot grain weight over non-inoculated grown relative effectiveness (percent STG: 165-16%; SSG: 283-14%) positive correlation yield activity. Furthermore, principal component analysis (PCA) categorical PCA clearly an revealing spp. than strain. documents equally effective sustaining well soil, but biosafety should be fully ensured before field release.