Purification and Identification by Immunological Techniques of Different Isoforms of Mammalian ATP Diphosphohydrolases

摘要: Apyrases or ATP diphosphohydrolases (ATPDases: EC 3.6.1.5) first mentioned by Meyerhof in 19451 refer to a family of enzymes that catalyse the hydrolysis γ and β phosphate residues triphospho- diphosphonucleosides. The original report Kalckar 19442 an ATPDase potato extracts initiated series studies on this enzyme (for reviews see 3, 4). Two isoforms have been described from Solanum tuberosum, varieties Desiree Pimpernel respectively5. ATPDases were later found other plant tissues: cabbage leaves, clover seeds, pea plumules, chick-pea roots, Alaska stems, Mimosa pudica pulvinius yellow lupin cotyledons3, 4. role plants has not yet clearly defined but implication starch synthesis proposed6. reported invertebrates including hematophagous insects, ticks leeches 4 7) parasitic worm8. In most these species, would act as antiplatelet agents preventing blood clotting3, 7. also exists prokaryotes9–11, among them, virulent Shigella where it is localised periplasmic space. It latter case, was suggested plays general cytotoxin involved directly indirectly actual killing host cells11.