Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research

摘要: In transmission electron microscopy (TEM), electrons are transmitted through a plastic-embedded specimen, and an image is formed. TEM enables the resolution visualization of detail not apparent via light microscopy, even when combined with immunohistochemical analysis. Ultrastructural examination tissues, cells microorganisms plays vital role in diagnostic pathology biologic research. used to study morphology their organelles, identification characterization viruses, bacteria, protozoa fungi. this protocol, we present method for preparing specimens obtained clinical or research settings, discussing particular requirements tissue cell preparation analysis, need rapid fixation possibility analysis already fixed formalin processed into paraffin blocks. Details fixation, embedding how prepare thin semi-thin sections, which can be complementary that performed ultimately using TEM, also described.