Comparison of Newcastle disease viruses isolated from cormorants in Canada and the USA in 1975, 1990 and 1992.

摘要: Seventeen Newcastle disease virus (NDV) isolates obtained from cormorants, turkeys, a pelican, and gull in Canada the USA collected 1975, 1990 1992 were analyzed for relatedness by monoclonal antibody profiling. In addition, nucleotide sequence analysis was performed two areas of fusion (F) gene 5 isolates. No difference antigenicity these 17 viruses, as determined binding patterns, seen. The amino acid sequences via sequencing at cleavage site F protein showed that all tested had pairs basic acids immediately upstream site, phenylalanine residue N-terminus F1 protein, which is consistent with velogenic NDV. deduced 6 virtually identical regardless species, year isolation, or location. However, 1975 cormorant isolate marked differences 1990-1992 signal region. These data indicate outbreaks caused same epizootic further suggest population NDV wild birds may be very stable. belief circulating cormorants transmitted into free-ranging turkey flocks located near North Dakota supported present study no distinction could made between viruses isolated turkeys birds.