Draft genome sequence of Bacillus paralicheniformis TRQ65, a biological control agent and plant growth-promoting bacterium isolated from wheat (Triticum turgidum subsp. durum) rhizosphere in the Yaqui Valley, Mexico.

摘要: The strain denominated TRQ65 was isolated from wheat (Triticum turgidum subsp. durum) commercial fields in the Yaqui Valley, Mexico. Here, we report its draft genome sequence, which presented ~ 4.5 million bp and 45.5% G + C content. Based on cutoff values species delimitation established for average nucleotide identity (> 95 to 96%), genome-to-genome distance calculator (> 70%), reference sequence alignment-based phylogeny builder method, strongly affiliated Bacillus paralicheniformis. rapid annotation using subsystem technology server revealed that contains genes related osmotic, oxidative stress response, as well auxin biosynthesis (plant growth promotion traits). In addition, antiSMASH BAGEL presence of involved lipopeptides antibiotic biosynthesis. function those annotated validated at a metabolic level, observing able tolerate saline (91.0%), water (155.0%) conditions, besides producing 28.8 ± 0.9 µg/mL indoles. showed inhibition (1.6 ± 0.4 cm zone) against causal agent spot blotch, Bipolaris sorokiniana. Finally, plant–microbe interactions assays confirm ability regulate growth, showing significant increment shoot height (26%), root length (40%), dry weight (48%), stem diameter (55%), biovolume index (246%). These findings provide insights future agricultural studies this strain.