Polymer-antibody fragment conjugates for biomedical applications

摘要: Abstract Many proteins benefit from improved solubility, immunocompatibility, pharmacokinetics or stability upon conjugation to polymers. For protein-conjugates used in delivery imaging vivo , this can mean increased efficacies due longer circulatory half-lives intracellular stability. In vitro polymers increase stability, reduce aggregation, mediate multimerization phase separation of assay sensitivities improve signal detection. The emergence recombinant antibody technologies over the past two decades has allowed relatively simple isolation (human) fragments, such as Fabs and single chain variable fragments (scFvs), that retain binding properties their parent molecules may exhibit additional reduced immunogenicity, tissue penetration packing density on sensor surfaces small sizes. addition, protein engineering approaches facilitate chemical functionalization have seen linked a broad spectrum chemically functionally diverse polymeric molecules. Of varied strategies polymer–protein coupling, amine cysteine are most widely applied chemistries with fragments. Simple poly(ethyleneglycol) half-life, decrease renal clearance, aggregation without compromising antigen binding. Meanwhile, be control coupling responsive enable context release drug payload polymer–antibody fragment complex. Recent years conjugates increasingly encroach into areas traditionally associated monoclonal antibody–polymers we discuss potential conjugates, applications delivery, engineering, diagnostics bioseparation.