Response of intracellular pH to acute anoxia in individual neurons from chemosensitive and nonchemosensitive regions of the medulla.
作者： Laura Chambers-Kersh , Nick A. Ritucci , Jay B. Dean , Robert W. Putnam
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摘要: The effect of acute (10 minutes) exposure to anoxia on intracellular pH (pHi) in individual brainstem neurons, slices from neonatal (P7 Pll) rats, was studied using a fluorescence microscopy imaging technique. Neurons 4 regions the medulla were studied, two which contained chemosensitive neurons (nucleus tractus solitarius, NTS, and ventrolateral medulla, VLM) did not contain (hypoglossal, Hyp, inferior olivary, IO). Acute caused rapid maintained acidification 0.1-0.3 unit that different vs. nonchemosensitive regions. Blocking contribution Na+/H+ exchange (NHE) pH, regulation by exposing presence inhibitor amiloride (1 mM) affect degree seen NTS VLM region, but significantly increased (to about 0.35 unit) Hyp IO neurons. In summary, anoxia-induced is between regions, NHE activity blunts latter These data suggest areas might have smaller acid load response than non-chemosensitive brainstem.
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