Cytocompatible gel formation of chitosan-glycerol phosphate solutions supplemented with hydroxyl ethyl cellulose is due to the presence of glyoxal.

摘要: To deliver and retain viable repair cells in a surgically prepared cartilage lesion, we previously developed an adhesive situ-gelling cell carrier by suspending solution of hydroxyethyl cellulose (HEC), which was then mixed with chitosan-glycerol phosphate to form chitosan-GP/HEC gel. The purpose this study elucidate the mechanism gelation maximally control gel time viability encapsulated cells. We analyzed role osmolality, pH, temperature, shrinkage, HEC. A chitosan-GP at pH 6.8 cytocompatible osmotic pressure (419 mOsm/kg) achieved lowering disodium GP concentration from 370 135 mM. This still thermogelling but only 73 degrees C. next discovered that glyoxal, common additive ether manufacturing, responsible for chitosan gelation. Monolayer survived proliferated up 1 mM however very narrow range glyoxal chitosan-GP/HEC, 0.1-0.15 mM, permitted formation, survival, proliferation. Chitosan gels containing HEC required slightly less solidify. Chitosan-GP/HEC loaded chondrocytes formed seal ex vivo mosaic arthroplasty defects sheep knee joints. In live sheep, bleeding occurred beneath part hydrogel carrier, cleared after month vivo. These data indicate is suitable as injectable delivery vehicle clinical orthopedic applications involving single use treatments guide acute processes.