Construction of Mouse Chromosome-specific DNA Libraries and Their Use for the Detection of X-ray-induced Aberrations
作者： J.J.W.A. Boei , A.S. Balajee , P. De Boer , W. Rens , J.A. Aten
DOI: 10.1080/09553009414550671
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摘要: We describe here the development of mouse chromosome-specific DNA libraries and their use in detection radiation-induced chromosome aberrations by fluorescence situ hybridization. Large metacentric chromosomes, resulting from a translocation involving chromosomes 1, 11 13, were flow-sorted. Using slit-scan technique for morphometric analysis, separated normal acrocentric aggregates. was amplified PCR using linker/adaptor method. In this pilot study, whole-body irradiated with 2 3 Gy scored metaphase spreads splenocytes cultured vitro. The results indicate that directly after radiation exposure, stable unstable are induced at about equal frequencies splenocytes. availability probes may prove very useful when analysing behaviour aberrations, as well testing many su...
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