Quantitative proteomics and phosphoproteomics of Trypanosoma cruzi epimastigote cell cycle

摘要: Abstract The protozoan Trypanosoma cruzi is the causative agent of neglected infectious illness Chagas disease. During its life cycle it differentiates into replicative and non-replicative stages. So far, T. cell division has been investigated by transcriptomics but not proteomics approaches. Here we show first quantitative proteome analysis division. epimastigote cultures were subject to synchronization with hydroxyurea harvested at different time points. Analysis flow cytometry, bright field fluorescence microscopy indicated that samples collected 0 h, 2 h, 6 h 14 h overrepresented G1, G1-S, S M phases, respectively. After trypsin digestion these samples, resulting peptides labelled iTRAQ subjected LC-MS/MS. Also, iTRAQ-labelled phosphopeptides enriched TiO2 access phosphoproteome. Overall, 597 protein groups 94 presented regulation most remarkable variation in abundance (S-phase). Comparison our proteomic data previous transcriptome-wise showed 16 sequence entries common, highest mRNA/protein correlation observed transcripts peak G1-phase. Our revealed regulated proteins which play important roles control other organisms some them previously detected nucleus or associated chromatin.