Use of an Lpp-OmpA fusion vehicle for bacterial surface display.

摘要: Publisher Summary This chapter discusses the application of lipoprotein (Lpp)–OmpA fusion vehicle to study surface bacteria. The Lpp–OmpA vector is designed for use in gram-negative bacterium Escherichia coli ; rationale its dibrid form stems from architecture E. envelope and properties major Lpp OmpA proteins. cells consists (i) a cytoplasmic membrane, (ii) periplasm, which peptidoglycan cell wall located, (iii) outer membrane (OM), unique structure composed proteins embedded an asymmetric lipid bilayer containing phospholipid inner leaflet lipopolysaccharide (LPS) leaflet. In Lpp-OmpA vector, domain serves target anchor tribrid OM required expression passenger. relevant these two follow immediately. protein is, therefore, synthesized with signal sequence exported, numerically most abundant coli, present as trimer, positioned such that located periplasm. A consisting first nine amino acids mature linked normally soluble, periplasmic TEM β -lactamase (Bla) was localized OM. ability terminus passenger utilized construct.