In Vitro Effects of Isoorientin, Forsythoside B, and Verbascoside on Bovine Kidney Cortex Glutathione Reductase
作者： Berivan Tandogan , Ayşegül Guvenc , Ihsan Calis , Nuriye Nuray Ulusu
DOI: 10.1002/KIN.20792
关键词:
摘要: Glutathione reductase (GR; E.C 1.6.4.2) catalyzes the reduction of oxidized glutathione to reduced by nicotinamide adenine dinucleotide phosphate (NADPH) dependent mechanism. GR participates in protection cell from toxic effects free radicals. In this study, we have investigated vitro isoorientin (C-glycosyl flavonoid), verbascoside, and forsythoside B (phenylethanoids) on purified bovine kidney cortex GR. These compounds present many plant species that been used as medicinal plants an interesting spectrum biological activity. We demonstrated these inhibit a concentration-dependent manner. Kinetic characterization inhibition is also done. Isoorientin noncompetitive inhibitor with respect both disulfide (GSSG) (KiGSSG 0.023 ± 0.001 mM) NADPH (KiNADPH 0.032 mM). Forsythoside acts uncompetitive GSSG 0.20 0.014 0.267 0.028 Verbascoside 0.242 0.024 0.175 0.016 Inhibition causes accumulation reactive oxygen depletion pool. enzyme may be significant drug resistance.
sci-hub.se 参考文章(21)
Ayşegül Güvenç, Yoshihito Okada, Esra Küpeli Akkol, Hayri Duman, Toru Okuyama, İhsan Çalış, None, Investigations of anti-inflammatory, antinociceptive, antioxidant and aldose reductase inhibitory activities of phenolic compounds from Sideritis brevibracteata Food Chemistry. ,vol. 118, pp. 686- 692 ,(2010) , 10.1016/J.FOODCHEM.2009.05.034
L. David Arscott, Donna M. Veine, Charles H. Williams, Mixed Disulfide with Glutathione as an Intermediate in the Reaction Catalyzed by Glutathione Reductase from Yeast and as a Major Form of the Enzyme in the Cell Biochemistry. ,vol. 39, pp. 4711- 4721 ,(2000) , 10.1021/BI9926431
Svenja MEIERJOHANN, Rolf D. WALTER, Sylke MÜLLER, Regulation of intracellular glutathione levels in erythrocytes infected with chloroquine-sensitive and chloroquine-resistant Plasmodium falciparum Biochemical Journal. ,vol. 368, pp. 761- 768 ,(2002) , 10.1042/BJ20020962
Holger Bauer, Karin Fritz-Wolf, Andreas Winzer, Sebastian Kühner, Susan Little, Vanessa Yardley, Hervé Vezin, Bruce Palfey, R. Heiner Schirmer, Elisabeth Davioud-Charvet, A Fluoro Analogue of the Menadione Derivative 6-[2‘-(3‘-Methyl)-1‘,4‘-naphthoquinolyl]hexanoic Acid Is a Suicide Substrate of Glutathione Reductase. Crystal Structure of the Alkylated Human Enzyme† Journal of the American Chemical Society. ,vol. 128, pp. 10784- 10794 ,(2006) , 10.1021/JA061155V
R. Luise Krauth-Siegel, L. David Arscott, Annette Schönleben-Janas, R. Heiner Schirmer, Charles H. Williams, Role of active site tyrosine residues in catalysis by human glutathione reductase. Biochemistry. ,vol. 37, pp. 13968- 13977 ,(1998) , 10.1021/BI980637J
Wolfgang Friebolin, Beate Jannack, Nicole Wenzel, Julien Furrer, Thomas Oeser, Cecilia P. Sanchez, Michael Lanzer, Vanessa Yardley, Katja Becker, Elisabeth Davioud-Charvet, Antimalarial Dual Drugs Based on Potent Inhibitors of Glutathione Reductase from Plasmodium falciparum Journal of Medicinal Chemistry. ,vol. 51, pp. 1260- 1277 ,(2008) , 10.1021/JM7009292
Hossein Nazemiyeh, M. Mukhlesur Rahman, Simon Gibbons, Lutfun Nahar, Abbas Delazar, Mohammed-Ali Ghahramani, Amir-Hossein Talebpour, Satyajit D. Sarker, Assessment of the antibacterial activity of phenylethanoid glycosides from Phlomis lanceolata against multiple-drug-resistant strains of Staphylococcus aureus. Journal of Natural Medicines. ,vol. 62, pp. 91- 95 ,(2007) , 10.1007/S11418-007-0194-Z
Esra Küpeli, Mustafa Aslan, İlhan Gürbüz, Erdem Yesilada, Evaluation of in vivo Biological Activity Profile of Isoorientin Zeitschrift für Naturforschung C. ,vol. 59, pp. 787- 790 ,(2004) , 10.1515/ZNC-2004-11-1204
Abbas Delazar, Araz Sabzevari, Mahdi Mojarrab, Hossein Nazemiyeh, Solmaz Esnaashari, Lutfun Nahar, Seied Mehdi Razavi, Satyajit Dey Sarker, Free-radical-scavenging principles from Phlomis caucasica. Journal of Natural Medicines. ,vol. 62, pp. 464- 466 ,(2008) , 10.1007/S11418-008-0255-Y
Berivan Tandogan, N. Nuray Ulusu, Purification and kinetics of bovine kidney cortex glutathione reductase. Protein and Peptide Letters. ,vol. 17, pp. 667- 674 ,(2010) , 10.2174/092986610791112684