Plasmid RFLP profiling and DNA homology in Thermus isolated from hot springs of different geographical areas

摘要: Thirty-four strains belonging to various species of the genusThermus (T. aquaticus, “T. thermophilus,” brockianus,” T. scotoductus, and genomic 2) isolated from hot springs different geographical areas were examined for plasmid content restriction fragment length polymorphism (RFLP) DNAs. The four numerical taxonomy cluster E 2 did not harbor DNA. Overall examination theHindIII-RFLP profiling DNA showed considerable variability between within species, with exception presumed clonal isolated. In spite this heterogeneity,HindIII digests a taxonomic gave subset fragments similar or identical length. Strains unclassified isolates S. Pedro do Sul that harbored (7 14 studied) exhibited strong homology regions. No homologous sequences these regions found in chromosomal which no plasmids detected. toT. scotoductus formed two groups, as estimated by probing coincided clusters proposed previously. Among other was also some strains. Strong same sources, suggesting are highly conserved present inThermus. For plasmid-containing strains, results RFLP profiling/DNA appear promising typing ofThermus at level biotypes individual namely, monitoring diversity frequency particular spring.