Proteomic Analyses Detect Higher Expression of C-Type Lectins in Imidacloprid-Resistant Colorado Potato Beetle Leptinotarsa decemlineata Say.

摘要: The Colorado potato beetle (CPB) is one of the most adaptable insect pests to both plant toxins and synthetic insecticides. Resistance in CPB reported for over 50 classes insecticides, mechanisms insecticide-resistance include enhanced detoxification enzymes, ABC transporters target site mutations. Adaptation insecticides also associated with changes behaviour, energy metabolism other physiological processes seemingly unrelated resistance but partially explained through genomic analyses. In present study, place genomics, we applied 2-dimensional (2-D) gel mass spectrometry investigate protein differences abdominal midgut tissue insecticide-susceptible (S) -resistant (R) CPB. proteomic analyses measured constitutive several proteins, highest match was identified as a C-type lectin (CTL), component innate immunity insects. expression CTL greater multi-resistant (LI) strain, same spot tissue. Exposure neonicotinoid insecticide, imidacloprid, increased found not laboratory (Lab) strain. No increase levels observed LI or field strain (NB) tolerant neonicotinoids. With exception biopesticides, such Bacillus thuringiensis (Bt), no previous studies have documented immune response by CTLs insects exposed fitness costs immune-related genes insecticide-resistant strains. This study demonstrates again how has been successful at adapting defenses well pathogens.