Molecular cloning of rat trkC and distribution of cells expressing messenger RNAs for members of the trk family in the rat central nervous system.

摘要: Tyrosine protein kinases trk, trkB and trkC are signal-transducing receptors for the neurotrophins nerve growth factor, brain-derived neurotrophin-3 neurotrophin-4. Here we report on isolation of cDNA fragments encoding a part rat trk irkB proteins, respectively, characterization full-length clone trkC. Cells expressing mRNAs different members family were identified in central nervous system by situ hybridization using oligonucleotide probes designed from isolated sequences complementary to mRNA coding extracellular region receptors. The expression was found be restricted neurons basal forebrain, caudate-putamen with features cholinergic cells magnocellular several brainstem nuclei. In contrast, widely distributed brain. Areas high levels or included olfactory formations, neocortex, hippocampus, thalamic hypothalamic nuclei, cerebellum spinal cord motoneurons. A similar distribution shown most areas but each probe specific these also provided distinct labeling patterns subregions, layers cells. Comparison between our data previous analyses low-affinity factor receptor suggests that modes action combinations mediate biological responses adult