Assessing accumulation and biliary excretion of naphthenic acids in yellow perch exposed to oil sands-affected waters.

摘要: Abstract Naphthenic acids are known to be the most prevalent group of organic compounds in oil sands tailings-associated waters. Yellow perch (Perca flavescens) were exposed for four months sands-influenced waters two experimental systems located on an lease 30 km north Fort McMurray Alberta: Demonstration Pond, containing tailings capped with natural surface water, and South Bison integrating lean sands. also sampled from three lakes: Mildred Lake that receives water Athabasca River, Sucker Lake, at edge extraction activity, Kimowin a distant reference site. measured muscle tissue using gas chromatography–mass spectrometry (GC–MS). Bile metabolites by GC–MS techniques high performance liquid chromatography (HPLC) fluorescence detection phenanthrene wavelengths. A method was developed chromatography–high resolution mass (LC–HRMS) evaluate naphthenic bile. Tissue analysis did not show pattern accumulation consistent concentrations LC–HRMS methods capable statistically distinguishing samples originating versus lakes. Although HPLC correlated, there no significant correlations these method. In yellow perch, sources do concentrate measurable amount excreted through biliary route. shown highly sensitive, selective promising technique as indicator exposure biota sands-derived acids.