Involvement of the CD95 (APO-1/Fas) receptor and ligand in liver damage.

摘要: Apoptosis occurs in the normal liver and various forms of disease. The CD95 (APO-1/Fas) (CD95) receptor mediates apoptosis, cells animal models are acutely sensitive to apoptosis initiated by this receptor. We have used primary human hepatocytes as a model system investigate CD95-mediated apoptotic damage. Treatment fresh with low concentrations agonistic antibodies against resulted > 95% cultured within 4 7.5 h. Immunohistology panel explanted tissues revealed that livers (n = 5) alcoholic cirrhosis 13) expressed constitutive levels CD95. expression was highly elevated hepatitis B virus-related 9) acute failure 8). By situ hybridization ligand messenger RNA absent but detected at high ongoing In cases hepatic failure, found primarily areas lymphocytic infiltration. contrast, patients damage, hepatocytes. These findings suggest destruction may involve killing T lymphocytes using receptor-ligand system. death might occur fratricide paracrine or autocrine mechanisms mediated themselves.