Essential roles for Cdx in murine primitive hematopoiesis.

摘要: The Cdx transcription factors play essential roles in primitive hematopoiesis the zebrafish where they exert their effects, part, through regulation of hox genes. Defects have also been reported mutant murine embryonic stem cell models, however, to date no mouse model reflecting hematopoietic phenotype has described. This is likely due, functional redundancy among members and early lethality Cdx2 null mutants. To circumvent these limitations, we used Cre-mediated conditional deletion assess impact concomitant loss Cdx1 on hematopoiesis. We found that Cdx1/Cdx2 double mutants exhibited defects yolk sac vasculature with reduced expression several genes encoding including Scl/Tal1. Chromatin immunoprecipitation analysis revealed Scl was occupied by vivo, differentiation could be rescued exogenous Scl. These findings demonstrate critical for upstream