Chapter 7. Tyrosine sulfation of HIV-1 coreceptors and other chemokine receptors.

摘要: Abstract Sulfotyrosines contribute to a number of critical extracellular protein–protein interactions, including the association HIV‐1 envelope glycoprotein with coreceptor CCR5, similar between Duffy binding protein Plasmodium vivax and antigen/receptor for chemokines, complement components C5a C3a their respective receptors, many CC‐ CXC‐chemokines receptors. In addition, antigen‐combining regions human antibodies include sulfotyrosines that are necessary antigen recognition. The study requires an array techniques, each its advantages limitations. These modulation tyrosyl‐protein sulfotransferase activity in mammalian cell lines, production tyrosine‐sulfated peptides direct chemical synthesis or enzymatic addition sulfate tyrosines cell‐culture cell‐free systems, use novel tRNA/tRNA‐synthetase pair capable introducing at specific sites into bacterially expressed proteins. Here we describe these various approaches role tyrosine sulfation chemokine receptors ligand entry.