Zooming, a Practical Strategy for Improving the Quality of Multidimensional NMR Spectra

摘要: Abstract A method has been developed, which is termed “zooming,” that provides high digital resolution in any region selected from an n -dimensional data set. The can be of arbitrary size (any number points). In the zooming approach as implemented “ZOOM” computer program, desired frequency-domain spectrum first extracted. Next, a “pseudo-FID” for constructed by inverse Fourier transformation. resulting time-domain block zero filled -dimensions and finally transformed back to frequency domain. basic principles behind are described, its application analysis 2D 3D NMR proteins demonstrated. Zooming expected even greater importance higher-dimensional where problems acquisition processing times storage space become more pronounced. implementation described here spectra consisting real or absolute-value data; at least one fill must have applied each dimension during initial data.