Chiral separation of 2-hydroxyglutaric acid on cinchonan carbamate based weak chiral anion exchangers by high-performance liquid chromatography.
作者： Carlos Calderón , Jeannie Horak , Michael Lämmerhofer
DOI: 10.1016/J.CHROMA.2016.05.042
关键词:
摘要: d- and l-2-Hydroxyglutaric acid (d- l-2-HG, respectively) are metabolites related to some diseases (2-hydroxyglutaric aciduria, cancer), which make their identification analysis crucially important for diagnostic purposes. Chiral stationary phases (CSP) based on tert-butylcarbamoyl-quinine -quinidine (Chiralpak QN-AX QD-AX), the corresponding zwitterionic derivatives ZWIX(+) Chiralpak ZWIX(-)) were employed in a weak anion-exchange mechanism perform enantiomer separation of l-2-HG without derivatization. QD-AX CSP showed most promising therefore optimization eluent, additives, temperature, required baseline solutes was carried out. Depending experimental conditions resolution values ranged up 2.0 with run times <20min MS-compatible conditions. Inversion elution order possible by using pseudo-enantiomeric CSP.
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