[76] The quantitative determination of mitochondrial hemoproteins

摘要: Publisher Summary This chapter focuses on the quantitative determinations of hemoproteins as found in mammalian mitochondria and preparations derived from these mitochondria. In such cases, may be mixed varying proportions. Mitochondria or mitochondrial are treated with bile salts to yield optically clear solutions. The describes determination cytochromes c+c l , Cytochrome b, c, direct cytochrome c 1 . concentrations α estimated spectrophotometrically after preferential reduction ascorbate. content b is turn dithionite. alone extraction into a water-ethanol-salt mixture. directly by ascorbate method which has been extracted.