Accurate identification of atypical Staphylococcus chromogenes plasma-clotting strains causing bovine mastitis

摘要: EnglishWe compared the potential of routine techniques used for identification Staphylococcus species, aiming to evaluate their accuracy in detection 43 chromogenes strains isolated from bovine mastitis that, despite being a coagulase-negative are able clot plasma. These could be mistakenly suspected S. aureus and lead an unappropriated treatment disease. MALDI-TOF, PCR-RFLP chaperonine gene groEL, sequencing 16S rRNA elongation factor Tu tuf were employed. Results four methods coincident only half because low groEL (51.2% accuracy). Even though all results identical, high MALDI-TOF (97.7% accuracy, with one strain misidentified) encourage use this technique, since it does not require laborious sample preparation, fast simple perform. Key words: chromogenes; mastitis; MALDI-TOF; tuf; portuguesNos comparamos o potencial de tecnicas rotineiras utilizadas para identificacao especies Staphylococcus, com objetivo avaliar acuracia delas na deteccao isolados envolvidos mastite bovina que, apesar ser uma especie coagulase-negativa, sao capazes coagular Essas cepas poderiam erroneamente suspeitas serem e levarem um tratamento nao adequado da doenca. do chaperonina sequenciamento fator elongacao Tu, tuf, foram avaliados. Os resultados dos quatro metodos coincidentes apenas metade das cepas, devido baixa precisao (51,2% acuracia). Apesar todos os identicos, alta (97,7% acuracia, cepa identificada incorretamente) encoraja uso dessa tecnica, pois, requer preparacao laboriosa amostras, sendo rapida simples executar. Palavras-chave: mastite; groEL.