Influenza Virus NS1 Protein Interacts with the Cellular 30 kDa Subunit of CPSF and Inhibits 3′ End Formation of Cellular Pre-mRNAs
作者： Martin E. Nemeroff , Silvia M.L. Barabino , Yongzhong Li , Walter Keller , Robert M. Krug
DOI: 10.1016/S1097-2765(00)80099-4
关键词:
摘要: Inhibition of the nuclear export poly(A)-containing mRNAs caused by influenza A virus NS1 protein requires its effector domain. Here, we demonstrate that domain functionally interacts with cellular 30 kDa subunit CPSF, an essential component 3' end processing machinery pre-mRNAs. In virus-infected cells, is physically associated CPSF kDa. Binding to in vitro prevents binding RNA substrate and inhibits cleavage polyadenylation host The also vivo, uncleaved pre-mRNA remains nucleus. Via this novel regulation processing, selectively cellular, not viral, mRNAs.
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