Mitochondrial DNA sequences and multiple data sets: a phylogenetic study of phytophagous beetles (Chrysomelidae: Ophraella).

摘要: This paper presents the phylogenetic infrastructure for an integrated historical and experimental study of host use evolution in chrysomelid leaf beetle genus Ophraella. We report collection sequence data from 16S ribosomal RNA (446 bp) cytochrome oxidase subunit I (420 mitochondrial genes 12 species Ophraella two outgroups. Sequence analysis revealed a strong A + T nucleotide bias, high interspecific COI divergences (up to 21.4%) that greatly exceeded those 5.9%), intraspecific 3.8%), dearth amino acid substitutions COI, differing substitution patterns stems loops. Intraspecific variation haplotypes generally supported genealogical coherence lineages, while suggesting cases paraphyletic species. Separate analyses sets yielded largely congruent trees. combined single shortest tree under maximum parsimony was identical trees provided by successive approximations, neighbor-joining, maximum-likelihood methods. topology proved robust various forms weighting most nodes were highly (by bootstrap analysis). mtDNA previously collected morphological electromorphic estimates all cladistic relationships except within one clade. Analysis pooled approach additionally support basal placement this clade, although remaining weakly incongruent with tree. Each set contained significantly structured signal respect exhibited limited conflict (character incongruence) each other. The set, however, found lack signal. These observations may imply pooling heterogeneously evolving classes obscured each, potential limitation approach.