Staphylococcal enterotoxin burden determines the type of T helper cell response and pathology of the maxillary sinus mucosa in rabbits.
作者： Hongqi Wei , Hui Yuan , Zhengwen Zhu , Zhiyong Liu , Jie Xin
DOI: 10.1016/J.INTIMP.2014.10.016
关键词:
摘要: Abstract Staphylococcal enterotoxin (SE) induces T lymphocyte activation along with nasal allergic inflammation during rhinosinusitis, but it is under debate on which types of helper (Th) cells respond exclusively or whether they synergically. We hypothesize that their responses may vary based dose SE. To test this hypothesis, we initiated to determine the nature cell response and pathological feature upon repeated exposure staphylococcal A (SEA) at different doses in maxillary sinus rabbits. SEA (0.6 60 ng) was instilled daily into left rabbits for 28 days. The right receiving normal saline used as control. Mucosal histological changes were examined by hematoxylin–eosin toluidine blue staining. Tissue expression myeloperoxidase (MPO), eosinophil cationic protein (ECP), T-box expressed (T-bet), GATA binding 3 (GATA-3) using immunohistochemistry. levels representative pro-inflammatory cytokines measured ELISA. 60 ng/day induced acute confirmed CT scan. Histopathologic examination revealed epithelial disruption, subepithelial edema, inflammatory infiltration. MPO T-bet expression, well interleukin (IL)-2 interferon (IFN)-γ levels, up-regulated. However, 0.6 ng/day did not cause discharge. Histological prominent eosinophilic ECP GATA-3 IL-4 IL-5 increased lower dose. In conclusion, rhinosinusitis associated a Th1-type immune high dose, predominantly Th2-biased low
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