Heterodera glycines utilizes promiscuous spliced leaders and demonstrates a unique preference for a species-specific spliced leader over C. elegans SL1

摘要: Spliced leader trans-splicing (SLTS) plays a part in the maturation of pre-mRNAs select species across multiple phyla but is particularly prevalent Nematoda. The role spliced leaders (SL) within cell unclear and an accurate assessment SL occurrence organism possible only after extensive sequencing data are available, which not currently case for many nematode species. discovery further complicated by absence sequences from high-throughput results due to incomplete 5’-ends transcripts during RNA-seq library preparation, known as 5′-bias. Existing datasets novel methodology were used identify both conserved SLs unique hypervariable Heterodera glycines, soybean cyst nematode. In H. twenty-one distinct found on 2,532 glycines transcripts. identified demonstrated high level promiscuity, meaning that some produced nine different individual SL-transcript combinations. Most uniquely, transcriptome analysis revealed first demonstrate higher rate using species-specific over well-conserved Caenorhabditis elegans SL-like sequences.