Acylation of subtilisin with long fatty acyl residues affects its activity and thermostability in aqueous medium
作者： Francisco J. Plou , Antonio Ballesteros
DOI: 10.1016/0014-5793(94)80415-X
关键词:
摘要: Subtilisin Carlsberg has been artificially hydrophobized by acylation with octanoyl or palmitoyl chlorides. Samples several degrees of substitution were obtained. Hydrophobization facilitates in some cases the binding synthetic natural substrates. Furthermore, derivatized subtilisins show improved thermal stability (15-fold at 45°C) aqueous solution. As a result, octanoyl-subtilisin exhibits enhanced thermostability without losing biological activity.
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