Genomic profiling reveals distinctive molecular relapse patterns in IDH1/2 wild-type glioblastoma.

摘要: Molecular changes associated with the progression of glioblastoma after standard radiochemotherapy remain poorly understood. We compared genomic profiles 27 paired primary and recurrent IDH1/2 wild-type glioblastomas by genome-wide array-based comparative hybridization. By bioinformatic analysis, tumor were normalized segmented, chromosomal gains losses identified taking cell content into account, difference deduced. Seven (26%) pairs lacked DNA copy number differences between tumors (equal pairs). The in 9/27 (33%) contained all imbalances plus additional ones, suggesting a sequential acquisition and/or selection for aberrations during (sequential In 11/27 (41%) pairs, divergent, i.e., but had lost others, polyclonal composition considerable clonal evolution (discrepant Losses on 9p21.3 harboring CDKN2A/B locus significantly more common from discrepant (nonequal) pairs. Nonequal showed ten regions 46 candidate genes recurrence. particular, numbers encoding apoptosis regulators frequently changed at progression. summary, approximately 25% have stable imbalances. contrast, 75% undergo further alter their upon recurrence impacting profile, process possibly facilitated loss tumor. © 2014 Wiley Periodicals, Inc.